Dietary Supplementation with Pithecellobium dulce (Roxb) Benth Fruits to Fattening Rabbits.

Pithecellobium dulce produces a fruit used in alternative medicine that could be utilized to feed rabbits. The objective of this study was to measure the effect of the P. dulce fruit on productive performance, carcass traits, meat characteristics, and meat product quality as well as shelf-life. Seventy-two California × English pot crossbreed rabbits (35 d age) were randomly distributed into two treatments: a control group without P. dulce and another group fed with 5% of P. dulce, and fattening for 28 d. Productive performance parameters, blood biochemistry and hematology, apparent digestibility, carcass traits, meat characteristics, and meat product shelf-life were measured. The results indicate inclusion of 5% P. dulce improves (p < 0.05) dry and organic matter digestibility and feed conversion rate, but some serum blood enzymes were increased (p < 0.05). The a* value, hardness, and pH decreased (p < 0.05) in the group fed with P. dulce. Antioxidant properties in the meatballs were different (p < 0.05), improving shelf-life and acceptance in sensory analysis. In conclusion, the use of 0.5% of P. dulce fruits to feed fattening rabbits can be used to improve the shelf-life of rabbit meat.

Nasal accumulation and metabolism of Δ9-tetrahydrocannabinol following aerosol ('vaping') administration in an adolescent rat model.

Passive aerosol exposure to Δ9-tetrahydrocannabinol (THC) in laboratory animals results in faster onset of action and less extensive liver metabolism compared to most other administration routes and might thus provide an ecologically relevant model of human cannabis inhalation. Previous studies have, however, overlooked the possibility that rodents, as obligate nose breathers, may accumulate aerosolized THC in the nasal cavity, from where the drug might directly diffuse to the brain. To test this, we administered THC (ten 5-s puffs of 100 mg/mL of THC) to adolescent (31-day-old) Sprague-Dawley rats of both sexes. We used liquid chromatography/tandem mass spectrometry to quantify the drug and its first-pass metabolites - 11-hydroxy-Δ9-THC (11-OH-THC) and 11-nor-9-carboxy-Δ9-THC (11-COOH-THC) - in nasal mucosa, lungs, plasma, and brain (olfactory bulb and cerebellum) at various time points after exposure. Apparent maximal THC concentration and area under the curve were ∼5 times higher in nasal mucosa than in lungs and 50-80 times higher than in plasma. Concentrations of 11-OH-THC were also greater in nasal mucosa and lungs than other tissues, whereas 11-COOH-THC was consistently undetectable. Experiments with microsomal preparations confirmed local metabolism of THC into 11-OH-THC (not 11-COOH-THC) in nasal mucosa and lungs. Finally, whole-body exposure to THC deposited substantial amounts of THC (∼150 mg/g) on fur but suppressed post-exposure grooming in rats of both sexes. The results indicate that THC absorption and metabolism in nasal mucosa and lungs, but probably not gastrointestinal tract, contribute to the pharmacological effects of aerosolized THC in male and female rats.

Mental Health Projects for University Students: A Systematic Review of the Scientific Literature Available in Portuguese, English, and Spanish.

The mental health of college students has been the source of research, projects, and public policies involving education, health, and psychology professionals. Having as its axis the study of mental health and the phenomenon of psychological illness, this systematic review aims to characterize mental health programs directed to college students, as well as the forms of interventions offered to reduce the incidence of psychological disorders. From the proposal, a survey was conducted in the databases Scopus, Lilacs, and the repository Alicia, in the period between 2010 and 2021, choosing the search phrase "Programas de saúde mental para universitários" in Portuguese, "Mental health projects for university students" in English, and "Proyectos en salud mental para estudiantes universitarios" in Spanish. The research areas spanned humanities and social sciences, with peer-reviewed and open access articles. The questions that instigated the study were as follows: What are the mental health problems affecting college students? What type of strategy has been adopted to map the demands of university students in relation to mental illness? How can the university space reorganize itself to work on interventional-preventive aspects, according to the studies? Initially, 740 203 articles were obtained, and after sifting through 13 productions, using the PRISMA systematization. Despite several research interrelating mental health and university space, most were dedicated to data collection, using questionnaires, inventories, and scales, standardized and non-standardized. Only three studies described intervention projects and programs to reduce the problems of psychological distress in college students. Unanimously, the investigations emphasize the need for monitoring the higher education population regarding mental health and, in parallel, the implementation of institutional public policies to meet the students' demands and reduce the rates of problems in the educational field.

Early adolescent subchronic low-dose nicotine exposure increases subsequent cocaine and fentanyl self-administration in Sprague-Dawley rats.

An exponential rise in nicotine-containing electronic-cigarette use has been observed during the period of adolescence. Preclinical studies have shown that nicotine exposure during early adolescence, but not adulthood, increases subsequent drug intake and reward. Although growing clinical trends highlight that stimulant use disorders are associated with the opioid epidemic, very few studies have assessed the effects of adolescent nicotine exposure on opioid intake. The objective of our current study is to develop a new animal model to assess the causal relationship of adolescent nicotine exposure on subsequent opioid intake. In this effort, we first replicate previous studies using a well-established 4-day nicotine paradigm. Rats are pretreated with a low dose of nicotine (2 × , 30 µg/kg/0.1 mL, intravenous) or saline during early adolescence (postnatal days 28-31) or adulthood (postnatal days 86-89). Following nicotine pretreatment on postnatal day 32 or postnatal day 90, animals underwent operant intravenous self-administration for the psychostimulant, cocaine [500 µg/kg/infusion (inf)] or the opioid, fentanyl (2.5 µg/kg/inf). We successfully show that adolescent but not adult, nicotine exposure enhances cocaine self-administration in male rats. Furthermore, we illustrate early adolescent but not adult nicotine exposure enhances fentanyl self-administration, independent of sex. Overall, our findings highlight that adolescence is a unique period of development that is vulnerable to nicotine-induced enhancement for cocaine and fentanyl self-administration in rats.

Fluoxetine for motor recovery after acute intracerebral hemorrhage, the FMRICH trial.

OBJECTIVES: Acute intracerebral hemorrhage (ICH) is a very common cause of disability. Previous evidence suggests that fluoxetine and other selective serotonin reuptake inhibitors improve, the recovery of motor function in patients with cerebral infarct. The purpose of this study was to investigate whether fluoxetine also improves motor recovery in patients with ICH. PATIENTS AND METHODS: This is a double blind, placebo controlled, multicenter randomized trial, patients recruited from three centers were assigned to receive 20 mg/day of fluoxetine or matching placebo for three months from within ten days after onset of symptoms. Primary outcome was change in Fugl-Meyer Motor Scale from baseline to day 90. RESULTS: Thirty patients (50 % women) were recruited to the fluoxetine (n = 14) or placebo (n = 16) groups. Median age was 55 years, the cause of the ICH was hypertension in 93.3 %, median volume of the hematomas was 22mm3. Basal ganglia hematoma was present in 67 % and, lobar location in 20 % of the patients. Improvement in FMMS at day 90 was significatively higher in the treatment group (median score 23) than in the placebo group, (median score 48), p = 0.001. No serious adverse events occurred. CONCLUSION: In addition to standard treatment, early prescription of fluoxetine was safe and helped to increase motor recovery 90 days after ICH. This finding adds to the evidence regarding its beneficial effect upon stroke related disability. Clinical Trial Registration-URL: http://www.clinicaltrials.gov. Unique identifier: NCT01737541.

Fertility, mortality, milk output, and body thermoregulation of growing Hy-Plus rabbits fed on diets supplemented with multi-enzymes preparation.

The aim of this study was to evaluate the fertility status, milk output, mortality, and body thermoregulation of rabbit does as affected by different levels of multi-enzyme extracts (EZ) in their diets. A total of 120 Hy-Plus rabbit does were divided into four comparable experimental groups (n = 30 does per group). Animals of each group were divided in six pens (five animals per pen), and each pen was used as an experimental unit. The first group was kept untreated and fed a commercial diet alone without enzyme extracts (EZ0), while the other groups were fed the same diet but supplemented with 1 (EZ1), 3 (EZ3), and 5 (EZ5) kg/ton of enzyme extracts, respectively. Feeding EZ additive increased (P < 0.05) conception and kindling rates, litter size and weight at birth, and litter size and bunny weight at weaning, with decreasing (P < 0.05) abortion rate. Moreover, total milk yield increased (P < 0.05) with increasing level of enzyme supplementation. Pre-weaning mortality decreased (P < 0.05) with EZ inclusion. Signs of vitality (rectal temperature, skin temperature, earlobe temperature, respiration rate, and pulse rate) were improved with EZ inclusion. For all results, 5 kg EZ/ton of feed was more effective than 1 and 3 kg EZ/ton feed. It can be concluded that supplementation of EZ in rabbit diet decreased mortality rate and enhanced fertility status and milk output.

Anti-inflammatory effect of aqueous extracts of spent Pleurotus ostreatus substrates in mouse ears treated with 12-O-tetradecanoylphorbol-13-acetate.

AIMS: To evaluate the application of spent Pleurotus ostreatus substrates, enriched or not with medicinal herbs, as a source of anti-inflammatory compounds. SUBJECTS AND METHODS: P. ostreatus was cultivated on five different substrates: Barley straw (BS) and BS combined 80:20 with medicinal herbs (Chenopodium ambrosioides L. [BS/CA], Rosmarinus officinalis L. [BS/RO], Litsea glaucescens Kunth [BS/LG], and Tagetes lucida Cav. [BS/TL]). The anti-inflammatory activity of aqueous extracts of spent mushroom substrates (SMSs) (4 mg/ear) was studied using an acute inflammation model in the mouse ear induced with 2.5 µg/ear 12-O-tetradecanoylphorbol13-acetate (TPA). RESULTS: Groups treated with BS/CA, BS/RO, and BS/LG aqueous extracts exhibited the best anti-inflammatory activity (94.0% ± 5.5%, 92.9% ± 0.6%, and 90.4% ± 5.0% inhibition of auricular edema [IAO], respectively), and these effects were significantly different (P < 0.05) from that of the positive control indomethacin (0.5 mg/ear). BS/TL and BS were also able to reduce TPA-induced inflammation but to a lesser extent (70.0% ± 6.7% and 43.5% ± 6.6% IAO, respectively). CONCLUSIONS: Spent P. ostreatus substrate of BS possesses a slight anti-inflammatory effect. The addition of CA L. to mushroom substrate showed a slightly synergistic effect while RO L. had an additive effect. In addition, LG Kunth and TL Cav. enhanced the anti-inflammatory effect of SMS. However, to determine whether there is a synergistic or additive effect, it is necessary to determine the anti-inflammatory effect of each medicinal herb.

Antiproliferative activity of epi-cercosporin in human solid tumor cell lines.

From cultures of Cercospora piaropi, a phytopathogenic fungus isolated from symptomatic leaves of water hyacinth was obtained a red compound, which, according to the spectroscopic data, was epi-cercosporin. It showed in vitro antiproliferative activity against the panel of human solid tumor cells HBL-100, HeLa, SW1573 and WiDr. Cell cycle studies revealed that epi-cercosporin induces accumulation of cells in G2/M phase.

Racism and mental health among university students in Mexico City.

OBJECTIVE: To assess whether differences exist in experiences of discrimination, perceptions of physical attractiveness, socioeconomic position, and mental health according to student's skin color. MATERIALS AND METHODS: The population included students from a public university in Mexico City. The following three groups were created based on skin color: white, light brown, and brown. By means of regression models, we evaluated whether differences in mental health according to skin color could be due to experiences of discrimination, perceptions of physical attractiveness, and/or socioeconomic position. RESULTS: Students with brown skin had lower levels of self-esteem and vitality and higher levels of fatigue and alcohol consumption. These differences were explained by more frequent experiences of discrimination and because students with brown skin perceived themselves as less attractive. CONCLUSIONS: This is one of the first studies to document the possible effects of racism on mental health among the urban population. Verification of these findings in representative samples is required.

Racism and mental health among university students in Mexico City / Racismo y salud mental en estudiantes universitarios de la Ciudad de México

OBJECTIVE: To assess whether differences exist in experiences of discrimination, perceptions of physical attractiveness, socioeconomic position, and mental health according to student's skin color. MATERIALS AND METHODS: The population included students from a public university in Mexico City. The following three groups were created based on skin color: white, light brown, and brown. By means of regression models, we evaluated whether differences in mental health according to skin color could be due to experiences of discrimination, perceptions of physical attractiveness, and/or socioeconomic position. RESULTS: Students with brown skin had lower levels of self-esteem and vitality and higher levels of fatigue and alcohol consumption. These differences were explained by more frequent experiences of discrimination and because students with brown skin perceived themselves as less attractive. CONCLUSIONS: This is one of the first studies to document the possible effects of racism on mental health among the urban population. Verification of these findings in representative samples is required.

OBJETIVOS: Conocer si existen diferencias en experiencias de discriminación, percepción del atractivo físico, posición socioeconómica y salud mental en función del color de la piel. MATERIAL Y MÉTODOS: La población consistió en estudiantes de una universidad de la Ciudad de México. A partir del color de piel se formaron tres grupos: blancos, morenos claros y morenos. Mediante modelos de regresión se evaluó si las diferencias en salud mental de acuerdo al color de piel se debían a experiencias de discriminación, percepción del atractivo físico y/o posición socioeconómica. RESULTADOS: Los que tenían piel morena tuvieron niveles más bajos de autoestima y vitalidad, pero más altos de cansancio y de consumo de alcohol. Estas diferencias fueron explicadas por las experiencias de discriminación y porque los estudiantes morenos se percibían menos atractivos. CONCLUSIONES: Por primera vez se documentan los posibles efectos del racismo en la salud mental en población urbana de México.

Percepción de enfermería en torno a la muerte de pacientes en etapa terminal / Perception by nursing staff about patients dealth with terminal illness

Introducción: La enfermera que se ubica en el área hospitalaria vive constantemente la muerte de pacientes. Objetivo: Identificar la percepción de la enfermera en torno a la muerte en pacientes terminales. Metodología: Fenomenológica, se utilizó muestreo teórico. Se realizaron 22 entrevistas a enfermeras que atendieron pacientes en etapa terminal a nivel hospitalario y que aceptaron ser entrevistadas en fecha sitio y hora elegida por ellas. Previo consentimiento informado se grabaron las entrevistas. Se manejó el supuesto "el personal de enfermería vive el proceso de muerte como un evento más, del trabajo cotidiano". Se realizó análisis de texto con el método de Giorgi. Hallazgos y análisis: Las enfermeras viven el proceso de muerte con miedo a la pérdida, manifestado con dolor, impotencia y tristeza. El dolor y la tristeza se relacionan con el tiempo de convivencia y lo que le significa el paciente. La impotencia de no poder hacer más por el paciente y finalmente no saber cómo apoyar a la familia. Estos sentimientos son manifestados con problemas de salud, como cansancio, agotamiento, situaciones que a la larga las convierte en pacientes potenciales porque se sienten sin redes de apoyo para resolver la pérdida. Conclusión: Las enfermeras viven el proceso de muerte de acuerdo a lo que le significa el paciente, a sus creencias sobre la muerte donde se enlazan la historia de vida, lo aspectos culturales y religiosos. Se les dificulta brindar atención de calidad dentro del aspecto psicológico.

Introduction: Nurses in hospitalization areas live constantly death of patients. Objective: To identify the nurses perception regarding the process of dying in patients with terminal illness. Methodology: Phenomenology methods. Theoretical sampling was used. 22 interviews were done to nurses who assisted patients with terminal illness at hospitalization areas, and who accepted being interviewed on date, time, and site chosen by them. Previous informed consent the interviews were recorded. It was used the premise of "nursing staff live the dying process as an event additional to the customary work". Analysis of text was done through the Giorgi's method. Findings and analysis: Nurses live the process of dying with fear to the lost, which is manifested by pain, incapability, and sadness. Pain and sadness feelings are related with the sharing time and what patient means to the nurse. Incapability of being able to do something else for the patient, and finally, not knowing how to support the family. These feelings are manifested by health problems, such as tiredness and fatigue; situations that after time make nurses become potential patients because they feel without a network of support to solve the lost. Conclusions: Nurses live the process of dying according to the meaning of the patient for them; to their beliefs about death where history of life is assorted; and to their own cultural and religious aspects. It is difficult for nurses to deliver quality of services within the psychological aspect.